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organelle constructs  (Addgene inc)


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    Addgene inc organelle constructs
    Organelle Constructs, supplied by Addgene inc, used in various techniques. Bioz Stars score: 92/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/organelle+constructs/DsRed2-Peroxisomes-4+(Plasmid+%2354503)/us12221491-1121-2-7
    Average 92 stars, based on 5 article reviews
    organelle constructs - by Bioz Stars, 2026-09
    92/100 stars

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    Construct:

    Article Title: An integrative systems genetic analysis of mammalian lipid metabolism
    Article Snippet: Cloning and expression vectors PCR amplification of genes of interest was performed from cDNA as previously described 58 using Phusion DNA polymerase (Thermo Fisher). .. Fluorescently tagged organelle constructs were purchased from AddGene (plasmid 1817: Lamp1-RFP, 54503: DsRed2-Peroxisomes-4 and 58014: mTa-gRFP-T-Endosomes-14). .. Amplified open-reading frames were cloned into expression vectors using Gateway Technology, restriction enzyme digest or Gibson assembly reactions (pDEST-47 GFP, C-term 3xFlag) or pAdEasy system (Agilent).

    Article Title: PSMD9 inhibitors for the treatment of hepatic lipid dysregulation
    Article Snippet: Expression vectors were sequence verified and subsequently amplified and purified by midi-prep (Promega) before use in downstream protocols. .. Fluorescently tagged organelle constructs were purchased from AddGene (plasmid #1817: Lamp1-RFP, #54503: DsRed2-Peroxisomes-4 and #58014: mTagRFP-T-Endosomes-14). ..

    Plasmid Preparation:

    Article Title: An integrative systems genetic analysis of mammalian lipid metabolism
    Article Snippet: Cloning and expression vectors PCR amplification of genes of interest was performed from cDNA as previously described 58 using Phusion DNA polymerase (Thermo Fisher). .. Fluorescently tagged organelle constructs were purchased from AddGene (plasmid 1817: Lamp1-RFP, 54503: DsRed2-Peroxisomes-4 and 58014: mTa-gRFP-T-Endosomes-14). .. Amplified open-reading frames were cloned into expression vectors using Gateway Technology, restriction enzyme digest or Gibson assembly reactions (pDEST-47 GFP, C-term 3xFlag) or pAdEasy system (Agilent).

    Article Title: PSMD9 inhibitors for the treatment of hepatic lipid dysregulation
    Article Snippet: Expression vectors were sequence verified and subsequently amplified and purified by midi-prep (Promega) before use in downstream protocols. .. Fluorescently tagged organelle constructs were purchased from AddGene (plasmid #1817: Lamp1-RFP, #54503: DsRed2-Peroxisomes-4 and #58014: mTagRFP-T-Endosomes-14). ..



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    Image Search Results


    (A) Inhibition of NAADP-AM stimulated calcium release by toremifene, mefloquine, posaconazole, and clarithromycin. Fibroblasts were loaded with NucBlue and Cal-520 (a calcium dye), treated with indicated drugs and then stimulated with NAADP-AM or HHBS. Cells showing Fmax/F0 > 2 (Fmax: maximum fluorescence intensity; F0: mean fluorescence intensity before stimulation) were counted as responsive cells. 150 cells were analyzed for each treatment. (B) Colocalization images of eGFP-Ebola with RFP-Lck (plasma membrane), RFP-Rab5a (early endosome), RFP-Rab7a (late endosome) or RFP-LAMP1 (lysosome). U2OS cells were treated with DMSO, EIPA, E-64D, U18666A, posaconazole, toremifene, mefloquine or clarithromycin (green: EBOV; red: organelle marker; blue: nuclei). (C and D) Drug effects on protease activities of cathepsin B and L. Recombinant cathepsin B or cathepsin L were treated with DMSO, Z-FA-FMK, cathepsin L inhibitor, toremifene, mefloquine, posaconazole, or clarithromycin. (E) Dose-response curves of toremifene (dark line), mefloquine (blue line), posaconazole (green line), and clarithromycin (purple line) in Amplex-red cholesterol assay in Hela cells. (F) Cholesterol accumulation induced by U18666A and posaconazole in fibroblasts (green: filipin; red: nuclei). (G) Lysosome enlargement induced by U18666A and posaconazole in fibroblasts (red: lysotracker; blue: nuclei). (H) Dose-response curves of toremifene (dark line), mefloquine (blue line), posaconazole (green line), and clarithromycin (purple line) in acid sphingomyelinase (ASM) assay in Hela cells. All experiments were performed in triplicate and data are representative of at least two independent experiments. Data are represented as mean±s.e.m.

    Journal: Antiviral research

    Article Title: Synergistic drug combination effectively blocks Ebola virus infection

    doi: 10.1016/j.antiviral.2016.11.017

    Figure Lengend Snippet: (A) Inhibition of NAADP-AM stimulated calcium release by toremifene, mefloquine, posaconazole, and clarithromycin. Fibroblasts were loaded with NucBlue and Cal-520 (a calcium dye), treated with indicated drugs and then stimulated with NAADP-AM or HHBS. Cells showing Fmax/F0 > 2 (Fmax: maximum fluorescence intensity; F0: mean fluorescence intensity before stimulation) were counted as responsive cells. 150 cells were analyzed for each treatment. (B) Colocalization images of eGFP-Ebola with RFP-Lck (plasma membrane), RFP-Rab5a (early endosome), RFP-Rab7a (late endosome) or RFP-LAMP1 (lysosome). U2OS cells were treated with DMSO, EIPA, E-64D, U18666A, posaconazole, toremifene, mefloquine or clarithromycin (green: EBOV; red: organelle marker; blue: nuclei). (C and D) Drug effects on protease activities of cathepsin B and L. Recombinant cathepsin B or cathepsin L were treated with DMSO, Z-FA-FMK, cathepsin L inhibitor, toremifene, mefloquine, posaconazole, or clarithromycin. (E) Dose-response curves of toremifene (dark line), mefloquine (blue line), posaconazole (green line), and clarithromycin (purple line) in Amplex-red cholesterol assay in Hela cells. (F) Cholesterol accumulation induced by U18666A and posaconazole in fibroblasts (green: filipin; red: nuclei). (G) Lysosome enlargement induced by U18666A and posaconazole in fibroblasts (red: lysotracker; blue: nuclei). (H) Dose-response curves of toremifene (dark line), mefloquine (blue line), posaconazole (green line), and clarithromycin (purple line) in acid sphingomyelinase (ASM) assay in Hela cells. All experiments were performed in triplicate and data are representative of at least two independent experiments. Data are represented as mean±s.e.m.

    Article Snippet: Constructs of RFP organelle marker were purchased from Thermo Fisher Scientific.

    Techniques: Inhibition, Fluorescence, Marker, Recombinant, Amplex Red Cholesterol Assay